General Cell Based Assays
Eurofins Discoverx PathHunter eXpress PTHR2 Total GPCR Internalization Assay, 10pl Kit. (93-0763E3CP0L)
The PathHunter PTHR2 Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of PTHR2 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged PTHR2 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the PTHR2 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR146 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0365E2ACP1L)
The PathHunter GPR146 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR146 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR146 and EA-tagged β-Arrestin-2. Activation of GPR146 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mINSRa Functional Assay, 10-Plate Kit. (93-1015E3CP16L)
The PathHunter eXpress mINSRa Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated mINSRa dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged mINSRa and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of the protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR85 U2OS β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0409E3CP1M)
The PathHunter GPR85 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR85 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR85 and EA-tagged β-Arrestin-2. Activation of GPR85 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Revvity Health Sciences Inc HTRF Human TNF-α / TNFR1 Binding Kit, 500 Assay Points
HTRF Human TNF-α / TNFR1 Binding Kit, 500 Assay Points
Eurofins Discoverx PathHunter eXpress LGR6 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0527E2ACP1L)
The PathHunter LGR6 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of LGR6 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged LGR6 and EA-tagged β-Arrestin-2. Activation of LGR6 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Echelon Biosciences Research Labs GENERIC PHOSPHATASE ASSAY KIT
Echelons Malachite Green Assay Kit is designed for the in vitro measurement of lipid and protein phosphatase activity. The liberated phosphate forms a colored complex with molybdate/malachite green, which is quantified by reading the absorbance at 620 nm. The assay uses water-soluble lipid substrates so there is no need to make phospholipid vesicles. The assay is fast, convenient and offers a flexible non-radioactive alternative for measuring specific phosphatase activities. The Malachite Green Assay kit can measure as little as 50 pmoles of free phosphate and has a high level of accuracy in the range of 200 to 2,000 pmol of phosphate per assay point. The kit includes enough reagents for 192 samples, standards, and controls. This item is non returnable
Meso Scale Discovery RPLEX HUM PRAS40 total ASY
The R-PLEX Human PRAS40 Total Assay Kit from Meso Scale Discovery is used to quantitatively measure levels of total PRAS40 protein in human cell and tissue samples The assay utilizes an electrochemiluminescent detection method and provides high sensitivity broad dynamic range and requires low sample input The kit includes a 96-well plate coated with capture antibody detection antibody read buffer sample diluent and vials of calibrator This assay allows for the simple and rapid analysis of PRAS40 protein levels which can provide insight into mTOR pathway biology and related disease states
Cayman Chemical TrnsglutamInase asy Stop10mL
Transglutaminase Assay Stop Solution has been tested and formulated to work exclusively with Cayman’s Transglutaminase Activity Assay Kit (Item No. 702250).
Eurofins Discoverx cAMP Hunter eXpress GALR1 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0058E2CP2L)
The cAMP Hunter GALR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. galanin) based activation of GALR1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GALR1, a naturally Gi/Go coupled GPCR. Upon activation of GALR1, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress CNR1 (CB1) CHO-K1 GPCR Assay, 10pl Kit. (95-0071E2CP2L)
The cAMP Hunter CNR1 (CB1) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CP 55,940) based activation of CNR1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CNR1, a naturally Gi/Go coupled GPCR. Upon activation of CNR1, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress LTB4R CHO-K1 GPCR Assay, 10-Plate Kit. (95-0085E2CP2L)
The cAMP Hunter LTB4R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. leukotriene B4) based activation of LTB4R GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type LTB4R, a naturally Gi/Go coupled GPCR. Upon activation of LTB4R, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Medchemexpress LLC Phospholipid transfer protein (human recombinant, His tag) | >95.0% | 50 UG
Recombinant human phospholipid transfer protein (PLTP) produced in HEK293 cells with a C-terminal His tag. Supplied as a purified protein for biochemical and cell-based studies of lipid transfer, lipoprotein remodeling, and modulation of inflammatory responses.
- Recombinant human protein expressed in HEK293 cells.
- C-terminal His tag for purification and detection.
- Purity >95% by reducing SDS-PAGE.
- Apparent molecular weight ~55-90 kDa due to glycosylation.
- Recommended reconstitution ≥100 μg/mL in ddH2O.
- Activity: 5 μg/mL inhibits ~87.3% of LPS-induced TNF-α secretion in RAW 264.7 cells.
- Store at -20°C; aliquot and freeze at -80°C for long-term storage.
Abcam Swinholide A, F-actin inhibitor, 50UG
MW 1389.9 Da, Purity >97%. Cell permeable, selective F-actin inhibitor. Sequesters and stabilizes actin dimers. Induces actin depolymerization and apoptosis. .
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter Human FGFR1-alpha-Klotho Functional Kinase Cell-Based Assay Kit (U2OS)
The PathHunter Human FGFR1-alpha-Klotho Stable Cell Line Functional Kinase Assay (U2OS) measures FGFR1-α-Klotho receptor tyrosine kinase activity via ligand-induced SH2 domain protein recruitment. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.